Is the same application that had problems with SPAM in the queue folder?
See: http://www.simple-talk.com/community/forums/thread/40932.aspx
If it is, then it's most likely to be that your server has been blocked by the ISP's for spam. I would check your IP address at
www.dnsstuff.com to see if it is listed in any blacklists etc.
Your server was left as an open relay (see: http://en.wikipedia.org/wiki/Open_mail_relay - you can also test your server here: http://www.abuse.net/relay.html), which is a spammers dream. This means they can send as much email (spam) as they like from your server. SMTP was left unprotected in IIS for some crazy reason, and it's the most common way of spammers getting the message out, without compromising themselves.
Regards
Greg